Validation of the micronucleus assay for biological dosimetry after high dose exposure
نویسندگان
چکیده
منابع مشابه
Cytokinesis blocked micronucleus (CB-MN) assay for biodosimetry of high dose accidental exposure
Background: Most of the cytogenetic dosimetry, except Premature Chromosome Condensation assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of simple CB-MN assay for prescreening of high dose accidental exposures. Materials and Methods: Human peripheral blood lymphocytes from two donors were gamma irradiated to different doses ranging from 0-15Gy, cultured for 72 h. Cytoch...
متن کاملAn Improved in Vitro Micronucleus Assay to Biological Dosimetry
The biological dosimetry is widely used to estimate the absorbed dose in people occupationally or accidentally exposed to the radiation for a better medical treatment, minimizing the harmful effects. Many techniques and methods have been proposed to detect and quantify the radioinduced lesions in genetic material, among them, the micronucleus (MN) assay. In the present study, we proposed an imp...
متن کاملCytokinesis blocked micronucleus (CB-MN) assay for biodosimetry of high dose accidental exposure
Background: Most of the cytogene c dosimetry, except Premature Chromosome Condensa on assay, saturates at 6 Gy. Present studies are aimed to assess feasibility of simple CB‐MN assay for prescreening of high dose accidental exposures. Materials and Methods: Human peripheral blood lymphocytes from two donors were gamma irradiated to different doses ranging from 0‐15Gy, cultured for 72 h. Cytochal...
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Biodosimetry is an essential tool for providing timely assessments of radiation exposure. For a large mass-casualty event involving exposure to ionizing radiation, it is of utmost importance to rapidly provide dose information for medical treatment. The well-established cytokinesis-block micronucleus (CBMN) assay is a validated method for biodosimetry. However, the need for an accelerated sampl...
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Background: Dosimetry is a fundamental part of the radiation processing industry. Aqueous inorganic dosimeters have been used for gamma radiation dosimetry such as standard ferrous sulfate dosimeter. The purpose of this study was to investigate the effect of gamma radiation on the diethyl fumarate DEF in dimethyl sulfoxide DMSO solution and its possible use in chemical dosimetry. Materials and ...
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ژورنال
عنوان ژورنال: Radioprotection
سال: 2008
ISSN: 0033-8451,1769-700X
DOI: 10.1051/radiopro:2008601